BioMed Research International / 2014 / Article / Tab 2

Research Article

Interactions of Neuropathogenic Escherichia coli K1 (RS218) and Its Derivatives Lacking Genomic Islands with Phagocytic Acanthamoeba castellanii and Nonphagocytic Brain Endothelial Cells

Table 2

RS218-derived islands (RDI’s) mutants and their interactions with Acanthamoeba castellanii. The number of E. coli K1 c.f.u. interacting with A. castellanii was considered as 100% and the results of mutants strains are expressed as the relative change.

IdentityA. castellanii
AssociationInvasionSurvivalCyst survival

K1 (RS218)100100100100
Plasmid cured RS218 130.49 ± 2526.19 ± 2*75.19 ± 10*25 ± 5*
RS218 in which black hole was filled with respective DNA from HB101143.8 ± 1932.74 ± 7*147.84 ± 23227.5 ± 23*
RDI 1111 ± 1296.42 ± 9132.8 ± 1493.75 ± 16
RDI 268.53 ± 7*36.90 ± 3*132.8 ± 1163.75 ± 8*
RDI 4246.53 ± 31143.44 ± 20*108.58 ± 1555 ± 5*
RDI 952 ± 3*76.19 ± 6*65 ± 8*10 ± 2*
RDI 1045.9 ± 6*51.78 ± 5*63.66 ± 6*8.75 ± 3*
RDI 1283.62 ± 1069 ± 11*92.18 ± 10158.75 ± 9
RDI 1488.92 ± 1581.54 ± 1197.26 ± 9165 ± 5
RDI 1560.19 ± 8*73.8 ± 12*110.34 ± 18136.25 ± 13
RDI 16124.7 ± 18116 ± 1193.35 ± 760 ± 9*
RDI 1749.95 ± 6*83.92 ± 9108.39 ± 16172.5 ± 12*
RDI 18117.97 ± 1628.57 ± 2*25.38 ± 3*8.12 ± 1*
RDI 21157 ± 14*39.28 ± 4*70.89 ± 6*45 ± 3*
RDI 22109.62 ± 938.69 ± 6*9.76 ± 1*16.25 ± 3*

(i) Association was calculated as follows: (number of bacterial c.f.u./number of amoebae) × 100 = bacterial association of A. castellanii.
(ii) Invasion was calculated as follows: (number of bacterial c.f.u./number of amoebae) × 100 = bacterial invasion of A. castellanii.
(iii) Survival was calculated as follows: (number of bacterial c.f.u./number of amoebae) × 100 = bacterial survival of A. castellanii.
(iv) Cyst survival was calculated as follows: (number of bacterial c.f.u./number of amoebae cysts) × 100 = bacterial survival of A. castellanii cysts.
*indicates a significant difference (P < 0.05 using paired t-test, one-tailed distribution) when data of mutant strains were compared with the wild type E. coli K1 data.

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