The Effects of Gene Polymorphisms in Interleukin-4 and Interleukin-6 on the Susceptibility of Rheumatoid Arthritis in a Chinese Population

Table 2

Primer pairs, PCR-RFLP analysis for IL-4 and IL-6 promoter polymorphisms.

SNP	Primer sequences	Annealing temperature (°C)	Amplification fragment (bp)	Restriction enzyme	Genotype bp	References
IL-4-590 C/T	5′-ACTAGGCCTCACCTGATACG-3′; 5′-GTTGTAATGCAGTCCTCCTG-3′	57	252	BsmFI	CC: 192, 60; CT: 252, 192, 60; TT: 252	[9]
IL-6-174 G/C	5′-GGAGTCACACACTCCACCT-3′; 5′-CTGATTGGAAACCTTATTAAG-3′	57	525	Hsp92II	GG: 327, 169; GC: 327, 169, 122; CC: 327, 122	[23]