Lipopolysaccharide Stimulates p62-Dependent Autophagy-Like Aggregate Clearance in Hepatocytes
Figure 5
LPS-induced p62-mediated autophagy involves signaling via MyD88/TIRAP and activation of NFκB. (a) Immunoblot of p62 in whole cell lysates from WT hepatocytes pretreated with control or TIRAP-siRNA prior to stimulation with LPS (100 ng/mL) for time points up to 16 h. (b) Immunoblot of p62 in whole cell lysates from WT hepatocytes pretreated with control or MyD88-siRNA prior to stimulation with LPS (100 ng/mL) for up to 16 h. (c) Immunoblot of p62 in whole cell liver lysates from MyD88WT or MyD88ko mice given no LPS, or LPS (5 mg/kg, ip) for 6 or 24 h. Each lane represents liver from one mouse (/gp). (d) WT hepatocytes were given LPS alone (100 ng/mL) or pretreated with NFκB inhibitor followed by LPS stimulation for up to 16 h. Nuclei were extracted from cells for EMSA for NFκB activation and whole cell lysates were collected from similarly treated groups of cells for p62 and LC3II immunoblot. Images representative of at least 3 separate experiments.
