Inhibition of Nek2 by Small Molecules Affects Proteasome Activity
Figure 7
Novel Nek2 inhibitor prevents Cdc2 and Cyclin B from being degraded by the proteasome, catching HeLa cells in G2/M phase. (a) Western blot showed that the degradation of Cdc2 and Cyclin B was inhibited by treatment of 10 nM HCI-2389. This effect was significant in Nek2 overexpressed HeLa cells. (b) Flow cytometry data showed that, treated by 10 nM HCI-2184 or 10 nM HCI-2389, around 50% HeLa cells stayed at G2/M phase. 100 nM nocodazole was used as the positive control.
