Potential Role of A2B Adenosine Receptors on Proliferation/Migration of Fetal Endothelium Derived from Preeclamptic Pregnancies
Figure 4
Stimulation of generates nitrotyrosine formation. (a) Representative images of western blots for endothelial nitric oxide synthase (eNOS, 130 kDa) and β-actin (43 kDa) in absence (−) or presence (+) of NECA (10 μM) and/or MRS-1754 (5 nM) during 12 hours in cells from normal (N) or preeclamptic pregnancies (PE). (b) Densitometry of the eNOS/β-actin ratio as showed in (a). (c) Nitrite concentrations in homogenate of cell after incubation (30 min) with NECA (10 μM) and/or MRS-1754 (5 nM). (d) Upper panel, representative images of western blots for nitration of tyrosine residues (Nitro-Y) in protein(s) at 55 kDa and β-actin (43 kDa). Bottom panel presents the densitometry of the nitrotyrosine/β-actin ratio in the analyzed groups as showed in the upper panel. In (c) and (d), and versus value in basal condition (i.e., without any treatment) of normal pregnancy or preeclampsia, respectively. Values are mean ± SEM. Values in respective column indicate . All experiments were performed in duplicate.
