Administration of the Resveratrol Analogues Isorhapontigenin and Heyneanol-A Protects Mice Hematopoietic Cells against Irradiation Injuries
Figure 5
REV, ISOR, and HEY-A increase the enzymatic activity of SOD2 and GPX1 in BMMNC. The cells received treatment with control, REV, ISOR, or HEY-A (1 μM) before being sham-irradiated as a control or irradiated with 2.0 Gy IR. The cells were incubated for 24 h before being analyzed. The expression of SOD2 and GPX1 mRNA was analyzed using qRT-PCR, and the enzymatic activities of SOD2 and GPX1 were analyzed using a SOD2 assay kit and a cellular GPX1 assay kit, respectively. ((a), (b)) Expression levels of SOD2 and GPX1 mRNA; ((c), (d)) enzymatic activity of SOD2 and GPX1 in BMMNC. Results of the mRNA-expression analysis and the enzymatic-activity analysis are expressed as means ± SE of changes in expression/activity in comparison with those of the control. versus control, versus vehicle, . REV: resveratrol; ISOR: isorhapontigenin; HEY-A: heyneanol-A; BMMNC: bone marrow mononuclear cells; IR: ionizing radiation.
