Neuroprotective Effects of Geniposide in SH-SY5Y Cells and Primary Hippocampal Neurons Exposed to Aβ42

Figure 1

A decrease in cytotoxicity of Aβ on rat hippocampal neurons in the presence of TLJN and its ingredients. Rat hippocampal neurons were cultured with the mixture of TLJN (high dose of TLJN: 5 μL TLJN per mL culture media (containing 83.5 μM geniposide and 8.35 μM ginsenoside Rg1); low dose of TLJN: 2 μL TLJN per mL culture media (containing 25.4 μM geniposide and 2.54 μM ginsenoside Rg1)) or its ingredient (geniposide (83.5 μM) and ginsenoside Rg1 (8.35 μM)) and Aβ (10 μM) for three days. The activities of LDH released in medium (a) were measured after the treatment. The survival rate of neurons was determined by immunohistochemical double staining with PI and Hoechst (b). The morphology of neurons stained with MAP2 and Hoechst were observed (c); bar = 50 μm. Data were represented as mean standard deviation (SD); ^*, ^**, and ^***.

(a)

(b)

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