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BioMed Research International
Volume 2014 (2014), Article ID 291271, 10 pages
http://dx.doi.org/10.1155/2014/291271
Research Article

In Vitro Antioxidant, Antiproliferative, and Phytochemical Study in Different Extracts of Nyctanthes arbortristis Flowers

Department of Biochemistry, School of Life Sciences, University of Hyderabad, Professor C. R. Rao Road, Gachibowli, Hyderabad, Andhra Pradesh 500 046, India

Received 28 February 2014; Revised 16 April 2014; Accepted 16 April 2014; Published 20 May 2014

Academic Editor: Aramati B. Reddy

Copyright © 2014 Manjulatha Khanapur et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nyctanthes arbortristis L. (Oleaceae) is widely used in the Indian system of traditional medicine and is reported to have various biological activities. The present study was intended to evaluate the antioxidant and antiproliferative activities of flower extracts of Nyctanthes arbortristis. The shade dried flowers were extracted with 95% ethanol under sonication and the antioxidant activities were investigated using in vitro assays along with the determination of phytochemical constituents (total polyphenol and total flavonoid). Arborside C and β-monogentiobioside ester of α-Crocetin were identified in crude active extracts through LCMS/MS analysis. The antiproliferative activity was carried out by MTT assay by employing different human cancer cell lines. The lowest IC50 value of 24.56 ± 6.63 μg/mL was observed against Colo 205 cell line. The extract exhibited significant antioxidant and antiproliferative properties and the observed biological activities in this study provide scientific validation of ethnomedicinal use of this plant.