Research Article
Transgenic Rat Model of Huntington’s Disease: A Histopathological Study and Correlations with Neurodegenerative Process in the Brain of HD Patients
| | Antibody | Host | Dilution | Source | Report |
| | Nestin | Rat monoclonal anti-mouse | 1 : 4 | DSHB | Progenitor cells marker | | Nestin | Mouse monoclonal
anti-human | 1 : 200 | Millipore | Progenitor cells marker | | β-III tubulin | Mouse monoclonal | 1 : 20 | Exbio | Neuronal marker | | MAP2 | Mouse monoclonal | 1 : 500 | Sigma-Aldrich | Neuronal marker | | MAP2 | Rabbit polyclonal | 1 : 700 | Millipore | Neuronal marker | | NeuN | Mouse monoclonal | 1 : 100 | Millipore | Marker of mature neurons | | Synaptophysin | Mouse monoclonal | 1 : 20 | Dako | Marker of neuronal synapses | | Vimentin (Cy3 conjugated) | Mouse monoclonal | 1 : 100 | Sigma-Aldrich | Astrocyte and radial glia marker | | NG2 | Rabbit polyclonal | 1 : 400 | Millipore | Oligodendrocyte precursor and pericyte marker | | APC | Mouse monoclonal | 1 : 200 | Calbiochem | Oligodendrocyte and astrocyte marker | | GFAP | Mouse monoclonal | 1 : 400 | Sigma-Aldrich | Astrocyte marker | | GFAP | Rabbit polyclonal | 1 : 400 | Dako | Astrocyte marker | | S100β | Mouse monoclonal | 1 : 1000 | Sigma-Aldrich | Astrocyte marker | | S100β | Rabbit polyclonal | 1 : 300 | Dako | Astrocyte marker | | Iba1 | Mouse monoclonal | 1 : 300 | Millipore | Microglia and macrophage marker | | PolyQ-huntingtin | Mouse monoclonal | 1 : 20000 | Millipore | Polyglutamine inclusions marker |
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