Trehalose-Based Eye Drops Preserve Viability and Functionality of Cultured Human Corneal Epithelial Cells during Desiccation

Figure 2

Immunocytochemical staining for active caspase 3 (red), actin (green), and nuclei (violet) in corneal epithelial cells after the treatment with tested preparations and 15 minutes of desiccation. Only in sample treated with Lacrimal (e) apoptotic cells were present; (a) Thealoz, (b) Hialeye 0.2, (c) Hialeye 0.4, (d) Keratostill, (e) Lacrimal, (f) Starazolin, (g) Systane, (h) 0.9% NaCl control, and (i) not treated control; magnification 200x; scale bar = 40 um.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)