BioMed Research International / 2014 / Article / Fig 1

Research Article

Sensitization of Cancer Cells through Reduction of Total Akt and Downregulation of Salinomycin-Induced pAkt, pGSk3β, pTSC2, and p4EBP1 by Cotreatment with MK-2206

Figure 1

High concentration of Sal reduced pAkt and total Akt levels in MK-2206-treated cells. (a) Hs578T cell extracts were collected at 24 h after treatment with 0.1 μM Sal (Sal-0.1), 5 μM Sal (Sal-5), 0.2 μM MK-2206 (MK-0.2), 0.5 μM MK-2206 (MK-0.5), or DMSO (Con). (b) Hs578T cell extracts were collected at 24 h after treatment with 0.5 μM MK-2206 (MK), 5 μM Sal (Sal), 0.5 μM MK-2206 with 5 μM Sal (MK + Sal), or DMSO (Con). (c) Hs578T cell extracts were collected at 24 h after treatment with 5 μM Sal (Sal), 0.2 μM MK-2206 (MK-0.2), 0.5 μM MK-2206 (MK-0.5), 1 μM MK-2206 (MK-1), 5 μM Sal with 0.2 μM MK-2206 (Sal + MK-0.2), 5 μM Sal with 0.5 μM MK-2206 (Sal + MK-0.5), 5 μM Sal with 1 μM MK-2206 (Sal + MK-1), or DMSO (Con). (d) Hs578T cell extracts were collected at 48 h after treatment with 1 μM MK-2206 (MK), 5 μM Sal (Sal), 1 μM MK-2206 with 5 μM Sal (MK + Sal), or DMSO (Con). The cells were used for Western blot analyses using antibodies against pAkt, Akt, C-PARP, pRb, and GAPDH.
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