Sensitization of Cancer Cells through Reduction of Total Akt and Downregulation of Salinomycin-Induced pAkt, pGSk3β, pTSC2, and p4EBP1 by Cotreatment with MK-2206

Figure 2

Cotreatment with MK-2206 and Sal increased pre-G1 regions in a dose-dependent manner. Hs578T cells were grown on 60 mm diameter dishes and treated with 0.2 μM MK-2206 (MK-0.2), 0.5 μM MK-2206 (MK-0.5), 1 μM MK-2206 (MK-1), 5 μM Sal (Sal), 0.2 μM MK-2206 with 5 μM Sal (MK-0.2 + Sal), 0.5 μM MK-2206 with 5 μM Sal (MK-0.5 +Sal), 1 μM MK-2206 with 5 μM Sal (MK-1 + Sal), or DMSO (Con). After 72 h, FACS analysis was performed as described in “Materials and Methods.”