BioMed Research International / 2014 / Article / Fig 3

Research Article

Sensitization of Cancer Cells through Reduction of Total Akt and Downregulation of Salinomycin-Induced pAkt, pGSk3β, pTSC2, and p4EBP1 by Cotreatment with MK-2206

Figure 3

Cotreatment with MK-2206 and Sal increased apoptosis in a time-dependent manner. (a) Hs578T cell extracts were collected at 24 h or 48 h after treatment with 0.5 μM MK-2206 (MK), 5 μM Sal (Sal), 0.5 μM MK-2206 with 5 μM Sal (MK + Sal), or DMSO (Con). The cells were used for Western blot analyses using antibodies against C-PARP and GAPDH. (b–d) Hs578T cells were grown on 6-well plates and treated with 0.2 μM MK-2206 (MK-0.2), 0.5 μM MK-2206 (MK-0.5), 1 μM MK-2206 (MK-1), 5 μM Sal (Sal), 0.2 μM MK-2206 with 5 μM Sal (MK-0.2 + Sal), 0.5 μM MK-2206 with 5 μM Sal (MK-0.5 + Sal), 1 μM MK-2206 with 5 μM Sal (MK-1 + Sal), or DMSO (Con). After 24 h, 48 h, or 72 h, all cells were then stained with Hoechst as described in “Materials and Methods.” The stained cells were subsequently examined using an inverted fluorescence microscope with a 32x objective lens.
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