Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity

Figure 6

Microarray data validation by quantitative real-time PCR (qRT-PCR). Validation of microarray data by qRT-PCR in MMG-incubated versus 1 g incubated PBLs. The results are consistent with the cumulative microarray data of miRNAs (a) and mRNAs (b). Values (fold change, dark grey bars) are means ± S.E. of expression levels calculated as the log2 (MMG/1 g) on PBL samples from 4 to 6 different donors. The value “1” of control 1 g PBLs (light grey bars) is arbitrarily given when no change is observed (^∗∗∗𝑃<0.001, ^∗∗𝑃<0.01, and ^∗𝑃<0.05, t-test).

(a)

(b)