Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration
Images show the cell morphology of hMSCs after 14 days (a). It reached confluence and formed a thick cell layer. Fluorescent images ((b)–(d)) show the progressive process of HUVECs networks on an hMSCs sheet after HUVECs were seeded on the hMSCs sheet for 2 hours (b), 1 day (c), and 7 days (d). Fluorescent images ((e)-(f)) show the morphology of HUVECs seeded on tissue culture plate after 2 hours (e) and 7 days (f). HUVECs on the tissue culture plate proliferated but did not form networks. (Scale bar = 100 μm).