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BioMed Research International
Volume 2014 (2014), Article ID 303451, 10 pages
Research Article

tasiRNA-ARF Pathway Moderates Floral Architecture in Arabidopsis Plants Subjected to Drought Stress

1Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
2National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan
3National Key Laboratory of Plant Cell Biotechnology, Agricultural Genetics Institute, Vietnamese Academy of Agricultural Science, Tu Liem, Hanoi, Vietnam
4Integrated Database Unit, Advanced Center for Computing and Communication (ACCC), RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
5Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa 244-0813, Japan
6Core Research for Evolutional Science and Technology, Japan Science and Technology, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

Received 22 May 2014; Revised 11 July 2014; Accepted 14 July 2014; Published 26 August 2014

Academic Editor: Hatem Rouached

Copyright © 2014 Akihiro Matsui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1. Distribution of smRNAs in abiotic stress-treated plants. smRNA sequences were classified based on where they mapped in the Arabidopsis genome (TAIR8). The circular graphs illustrate the distribution of mapped smRNA sequences obtained from plants subjected to each different treatment. The miRNAs and ta-siRNAs indicate that smRNA sequences are derived from MIRNA loci and TAS loci. Small RNAs mapped to regions coding for proteins were classified into two groups, smRNAs mapped to the sense strand and smRNAs mapped to the antisense strand of protein-coding genes. Transposable element (TE) genes and pseudogenes are AGI coded genes annotated as TEs and pseudo genes, respectively. Intergenic TEs represent smRNAs mapped to the TEs in intergenic regions.

Supplemental Figure 2. Deep sequencing of miRNAs and ta-siRNAs in non-stressed and stress-treated plants. The number of RNA sequences was normalized by dividing by the total number of obtained miRNA sequences. P-values were calculated using an enrichment test on each miRNA fraction, based on their hypergeometric distribution. Asterisk in the table indicates the p-value was lower than 10-3. Previously reported stress- upregulated or downregulated smRNAs are shown in red or blue, respectively.

Supplemental Figure 3. Seed production in ta-siRNA biosynthesis mutants, rdr6, sgs3, dcl4 and ago7, subjected to drought stress, and in rdr6 and ARF3pro:ARFmut mutants subjected to high-salinity stress. (a) Soil water content during the course of the imposed drought treatment. (b) Seed numbers in the 1st silique (n =12). (c) Seed number in the 1st silique of plants subjected to a drought stress after artificial pollination and self-pollination (n =12). (d) Seed numbers in the 1st silique of three-week-old plants grown in soil and treated with 100 mM NaCl for one week (n =12). (e) Short siliques were observed in rdr6 and ARF3pro:ARF3mut plants subjected to high-salinity stress. (f) Short stamens were observed in rdr6 and ARF3pro:ARF3mut plants subjected to high-salinity stress.

Supplemental Figure 4. Scatter plot analysis for drought stress-responsive expression in rdr6 and wild-type plants. (a) Scatter plot analysis for drought stress-responsive expression of water deprivation response-related genes (GO:0009414) in rdr6 and wild-type plants. Horizontal axis represents log2 ratio of (drought / non-stressed) in wild-type plants. Vertical axis represents log2ratio of (drought / non-stressed) in rdr6 mutants. (b) Line graph for expressions of YUCCA genes. Horizontal axis represents normalized intensities of microarray probes targeting YUCCA genes.

Supplemental Table 1. List of primer sequences.

Supplemental Table 2. Differentially expressed genes in flower buds of rdr6 and wild-type under drought stress treatment and untreatment.

Supplemental Table 3. Expression profiles of floral organ development-related genes (GO:0048437)

Supplebmental Table 4. Expression profiles of 155 genes co-expressed with ARF3 and ARF4.

  1. Supplementary Material