Bioactivity-Guided Fractionation Identifies Amygdalin as a Potent Neurotrophic Agent from Herbal Medicine Semen Persicae Extract
Bioactivity-guided fractionation for the identification of the neurotrophic agent from Semen Persicae extract. (a) Scheme illustrating the bioactivity-guided fractionation procedure for the isolation of botanical glycoside amygdalin as the neurotropic compound from Semen Persicae extract. The structure of amygdalin was generated by ChemSketch software (http://www.acdlabs.com). (b, upper panel) Assay of the fractions generated by liquid-liquid extraction. The aqueous Semen Persicae extract was sequentially extracted by ethyl acetate, n-butanol, giving rise to three fractions: ethyl acetate extract, n-butanol extract, and H2O phase. These fractions were assayed for the induction of neurite outgrowth in PC12 cells. The images were acquired under a microscope. (b, lower panel) Assay of the fractions generated by semipreparative HPLC separation and representative images are shown. The n-butanol extract was separated into a total of 9 fractions by semipreparative HPLC isolation. All HPLC fractions were assayed for the induction of neurite outgrowth in PC12 cells. The images were acquired under a microscope and F5 with neurotrophic activities are shown. (c) ESI-MS analysis and chemical information of the active compound. ESI-MS ions () were identified by HPLC-MS analysis as described in Section 2. The ultraviolet (UV) absorption spectrum was recorded by HPLC UV detector for the identification of UV (nm).
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