BioMed Research International / 2014 / Article / Fig 4

Research Article

Bioactivity-Guided Fractionation Identifies Amygdalin as a Potent Neurotrophic Agent from Herbal Medicine Semen Persicae Extract

Figure 4

Evidence for the neurotrophic activity of amygdalin. (a) The neurotrophic activity of amygdalin. PC12 cells were treated with amygdalin at concentration ranging from 0 to 20 μM for 72 h. The neurite outgrowth was examined under a microscope. The representative images were shown. (b) Quantitative analysis of amygdalin-induced neurite outgrowth. Data are presented as mean ± SD ( ). (Treatment versus Control). (c) Effect of amygdalin on the cell viability. PC12 cells were treated as described in Panels (a) and (b). The cell viability was determined by standard MTT assay ( ). (d) Effect of amygdalin on the expression of neuronal biomarkers. At the end of drug treatment, neuronal biomarkers were detected by Western blotting as described in Section 2. Three independent experiments were performed. The mean values for fold of induction were shown under each treatment.

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