BioMed Research International / 2014 / Article / Fig 6

Research Article

Bioactivity-Guided Fractionation Identifies Amygdalin as a Potent Neurotrophic Agent from Herbal Medicine Semen Persicae Extract

Figure 6

Potential role of ERK1/2 in the neurotrophic activity of amygdalin. (a) Effect of MEK inhibitor PD98059 on the activation of ERK1/2 by amygdalin and Semen Persicae extract. PC12 were incubated with amygdalin (20 μM) or Semen Persicae extract (1 mg/mL) at the presence or absence of PD98059 (20 μM) for 60 minutes. Total and phosphorylated ERK1/2 were detected by Western blotting analysis. (b) Effect of MEK inhibitor PD98059 on the neurotrophic activity of amygdalin and Semen Persicae extract. After 3 days of incubation with amygdalin (20 μM) or Semen Persicae extract (1 mg/mL) at the presence or absence of PD98059 (20 μM), the cells were stained with FITC-phalloidin for F-actin, anti-MAP2 for neuronal characteristics, and DAPI for cell nucleus. The images were acquired under a fluorescence microscope.
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