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BioMed Research International
Volume 2014, Article ID 308613, 11 pages
Research Article

Immobilization of a Pleurotus ostreatus Laccase Mixture on Perlite and Its Application to Dye Decolourisation

1Dipartimento di Scienze Chimiche, Università di Napoli Federico II, via Cynthia 4, 80126 Napoli, Italy
2Istituto di Ricerche sulla Combustione, Consiglio Nazionale delle Ricerche, Piazzale V. Tecchio 80, 80125 Napoli, Italy
3Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy

Received 13 January 2014; Revised 3 April 2014; Accepted 24 April 2014; Published 8 May 2014

Academic Editor: Neelu Nawani

Copyright © 2014 Cinzia Pezzella et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In the present study, a crude laccase preparation from Pleurotus ostreatus was successfully immobilized on perlite, a cheap porous silica material, and tested for Remazol Brilliant Blue R (RBBR) decolourisation in a fluidized bed recycle reactor. Results showed that RBBR decolourisation is mainly due to enzyme action despite the occurrence of dye adsorption-related enzyme inhibition. Fine tuning of immobilization conditions allowed balancing the immobilization yield and the resulting rate of decolourisation, with the adsorption capacity of the solid biocatalyst. In the continuous lab scale reactor, a maximum conversion degree of 56.1% was achieved at reactor space-time of 4.2 h. Stability and catalytic parameters of the immobilized laccases were also assessed in comparison with the soluble counterparts, revealing an increase in stability, despite a reduction of the catalytic performances. Both effects are most likely ascribable to the occurrence of multipoint attachment phenomena.