Characterization of Glial Cell Models and In Vitro Manipulation of the Neuregulin1/ErbB System

Figure 4

Western blot analysis confirms that primary cultures and cell lines derived from OEC and SC express different levels of ErbB receptors and glial proteins. Western blot analysis of proteins extracted from OEC, NOBEC, RT4-D6P2T, and SC were analyzed with antibodies directed to ErbB1, ErbB2, ErbB3, ErbB4, NRG1, p75, and GAPDH. Different NRG1 isoforms are expressed by OEC, NOBEC, and SC. An asterisk indicates a positive control for ErbB4 expression (a cell line stably expressing ErbB4 [18]).