Characterization of Glial Cell Models and In Vitro Manipulation of the Neuregulin1/ErbB System
Figure 5
Recombinant ecto-ErbB4-FLAG is expressed and released by cells and is able to interact with soluble NRG1. (a) The correct expression of ecto-ErbB4-FLAG was assayed both in the cell extract and in the conditioned medium (surnatant) of transiently transfected COS7 cells (ecto-ErbB4). Mock samples are COS7 cells transfected with the empty vector. The asterisk indicates an unspecific band. (b) Different amounts of soluble recombinant NRG11 tagged with 6 histidine were incubated with conditioned medium of COS7 cells transiently expressing recombinant ecto-ErbB4-FLAG. A FLAG coimmunoprecipitation was performed, followed by Western blot against histidine to recognize NRG11. The asterisk indicates the band corresponding to the primary antibody used for coimmunoprecipitation.
