Characterization of Glial Cell Models and In Vitro Manipulation of the Neuregulin1/ErbB System
Figure 6
NOBEC transiently expressing ecto-ErbB4 and ecto-ErbB3 migrate more than control cells and their migration is inhibited by DAPT treatment. Migration activity of NOBEC transiently transfected with empty vector (AAV) was compared with migration activity of NOBEC expressing ecto-ErbB4 (ErbB4, Panel (a)) or ecto-ErbB3 (ErbB3, Panel (b)). Soluble recombinant NRG11 stimulates NOBEC wild type (WT) migration (Panel (c)). NOBEC wild type (Panel (d)) or NOBEC expressing ecto-ErbB4 (Panel (e)) were pretreated for three days with 100 M DAPT (-secretase inhibitor) or DMSO (mock control). Cell migration was assessed by Transwell assays. Values represent the average of three biological replicates performed as technical triplicates. Values of each replicate are expressed in percentage with respect to the total number of cells that migrated in that experiment (**, ; ***, ).
