Andrographolide Induces Apoptosis of C6 Glioma Cells via the ERK-p53-Caspase 7-PARP Pathway

Figure 1

The structure of AND and the effect of AND on the survival of C6 glioma cells. (a) The chemical structure of AND. (b) The cells were treated with 0.1% dimethyl sulfoxide (DMSO) (CTL), 5, 10, 15, or 20 μM of AND for 24 h, and cell viability was determined using the MTT assay. 𝑁=3. ^∗∗𝑃<0.01, compared to the control group. (c) Flow cytometric analysis of AND-induced apoptosis. Cells were treated with 15 μM AND for different intervals and stained with annexin V and propidium iodide PI for flow cytometric analysis.

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