Andrographolide Induces Apoptosis of C6 Glioma Cells via the ERK-p53-Caspase 7-PARP Pathway

Figure 6

Effect of AND on cell viability and the expression of pERK in normal cultured rat astrocytes and C6 glioblastoma cells. (a) Cell survival analysis. Normal astrocytes were treated with 0.1% DMSO (CTL), 5, 10, 15, or 20 μM of AND for 24 h, and the cell viability was determined by MTT assay. 𝑁=3. (b) Blot analysis. Astrocytes and C6 cells were treated with 15 μM AND for 0, 6, 12, or 24 h, and cell lysates were analyzed for pERK and ERK (upper panel). The quantization of p-ERK1, p-ERK2, ERK1, and ERK2 was presented in the following plots (lower panel). 𝑁=3. ^∗𝑃<0.05, ^∗∗𝑃<0.01, as compared to the 0 h group.

(a)

(b)