In Vitro Antimicrobial and Antiprotozoal Activities, Phytochemical Screening and Heavy Metals Toxicity of Different Parts of Ballota nigra

Table 2

Antibacterial activity of Ballota nigra stem.

Test microorganisms	Zone of inhibition (mm)
	Crude		n-Hexane		Chloroform		Ethyl acetate		n-Butanol		Aqueous		Standards
Crude extract/fractions (𝜇g/𝜇L)	2/1	2/1	2/1	2/1	2/1	2/1	2/1	2/1	2/1	2/1	2/1	2/1	Amx*	Lev**
Salmonella typhi	11	13	—	—	12	12	15	15	08	09	—	—	—	28
Staphylococcus aureus	10	09	16	15	19	18	16	15	07	07	—	—	—	29
Proteus mirabilis	14	14	—	—	18	16	13	11	08	08	—	—	—	26
Klebsiella pneumoniae	15	13	—	—	09	09	10	10	—	—	08	07	—	28
Escherichia coli	08	09	12	12	10	09	—	—	—	—	10	12	—	22
Enterococcus faecalis	14	12	—	—	09	10	13	11	—	—	—	—	—	29

(—): no inhibition zone (7 mm), *amoxicillin: 5 𝜇g/𝜇L, and **levofloxacin: 5 𝜇g/𝜇L.