In Vitro Antimicrobial and Antiprotozoal Activities, Phytochemical Screening and Heavy Metals Toxicity of Different Parts of Ballota nigra

Table 5

Leishmania parasites in RPMI 1640 media and negative control (DMSO).

Media	Culture growth after days
	1st	2nd	3rd	4th	5th	6th	7th	8th	9th	10th	11th
RPMI (1640)	−	−	−	−	+	+	++	++	++	++	++
Negative control	−	−	−	−	−	−	−	−	−	−	−

(−) sign indicates amastigote form of cutaneous leishmaniasis; (+) indicates initial conversion of amastigote into promastigotes; and (++) shows promastigotes growth at peak level.