Highly Effective Renaturation of a Streptokinase from Streptococcus pyogenes DT7 as Inclusion Bodies Overexpressed in Escherichia coli

Table 1

Effect of surfactant, glycerol, and temperature on the renaturation of cell lysate E. coli/pESK.

Parameter	SK activity (U/mL)
	at 37°C for 1 h	at 37°C for 1 h + 10% glycerol	at 4°C for 6 h + 10% glycerol
0.5% Triton X-100	643.4 ± 3.6	5574.0 ± 36.2	7591.3 ± 45.2
1% Tween 20	657.6 ± 5.7	4592.8 ± 27.1	6587.7 ± 27.1
1.5% Tween 80	691.6 ± 6.6	5226.8 ± 50.6	6578.7 ± 63.3
No surfactant	182.1 ± 1.8	184.3 ± 3.6	189.4 ± 3.6