Wnt Pathway Activation in Long Term Remnant Rat Model
Figure 2
Expression of the canonical Wnt signaling components in rat 5/6 renal mass reduction model. mRNA levels of the Wnt ligands Wnt1, Wnt3a, and Wnt10a (canonical), Wnt5a (noncanonical), and Wnt7b (Ca2+ pathway); the Wnt receptors FZD1, 2, 3, 6, and 8 and LRP5; intracellular signal transducers β-catenin (β-Cat) and glucose sintase kinase3-β (GSK-3β); and the target genes axin2, lymphoid enhancer-binding factor 1 (LEF1), TCF4, and WISP1 were assessed in rat kidneys of sham animals (S) and 5/6 mass reduction animals (Nx) by quantitative real-time PCR (qRT-PCR). Results are derived from 8-9 animals per group after 12 weeks (white columns) and 18 weeks (grey columns) after the surgery. Columns show median of log-fold change for each gene. # and ###, two-tailed Student’s -test for unpaired observations between S and Nx groups. and , two-tailed Student’s -test for unpaired observations between 12 and 18 weeks.
