Wnt Pathway Activation in Long Term Remnant Rat Model

Figure 5

Expression and localization of β-catenin of representative kidneys in Nx rats. (a) Costain against FBPase in pink (Vulcan fast red), specific marker of proximal tubule (PT), and ATPase in brown (DAB), specific marker of distal tubule (DT), are made in serial cuts to discriminate between tubules; in (b) and (d) β-catenin stain was made in rat kidneys in pink (Vulcan fast red) and nuclei in blue (hematoxylin). Higher magnifications of tubular are shown in (c) and (e); (d) showing distal tubules with the translocated nuclei (arrows); (e) showing magnification of two proximal tubules with apical localization of β-catenin and one distal tubule with membrane localization, and short arrows indicate β-catenin translocated nucleus. As the β-catenin stain was made with Vulcan fast red, when slices were observed with a Texas Red filter clear image of translocated nuclei was obtained and false positives could be discarded; short arrow shows the confirmed 29 β-catenin translocated nucleus (f). Representative pictures with focus on the cortex area are given. Quantification of nuclei translocated β-catenin was done, related to proximal and distal epithelium surface. Stains are representative of using at least six different animals per group (magnification as indicated).