Comparative Metagenomic Analysis of Human Gut Microbiome Composition Using Two Different Bioinformatic Pipelines

Figure 2

Family level taxonomic composition correlated with the number of OTUs identified by Mg-Rast and QIIme. Two distinct trends are shown, respectively, for both the tools. Different colors represent different Phyla that belong to the identified families. Qiime reported higher general values for each family compared to Mg-Rast. Seven families were identified with a widely different score (>1000 sequences, indicated by bars): Bacteroidaceae (belongs to Bacteroidetes Phylum, in yellow); Streptococcaceae, Clostridiaceae, and Lachnospiraceae (belong to Firmicutes Philum, in purple); Alcaligenaceae, Enterobacteriaceae, and Pasteurellaceae (belong to Protecobacteria Philum, in orange). No hits field (in gray) represents those sequences who were not assigned to the Bacteria root (605 for Mg-Rast and 12 for QIIme). Unclassified field (in green) represents those sequences who belong to the Bacteria root but both the tools were unable to identify precise taxonomy.