Parkia biglobosa Improves Mitochondrial Functioning and Protects against Neurotoxic Agents in Rat Brain Hippocampal Slices
Figure 5
Effect of PBE and catechin on nonprotein thiol content. Hippocampal brain slices were preincubated with P. biglobosa extract, PBE (25, 50, 100, or 200 g/mL), or catechin (1, 5, or 10 g/mL) for 30 min before final incubation with toxicants (SNP, , or ) for 1 h. Slices homogenates were deproteinized and the free –SH groups were quantified in the protein-free supernatant by measuring the intensity of the color produced on reacting with Ellman’s reagent at 412 nm. Data are expressed as mean ± SEM of three independent experiments. # and ## versus untreated slices (control) and and versus slices treated with toxicants (SNP, , or ) alone, as determined by one-way ANOVA followed by the Newman-Keuls multiple comparison test.
