Mass Spectrometric Analysis of Differentially Expressed Proteins in an Endangered Medicinal Herb, Picrorhiza kurroa

Figure 5

This image shows the connecting link of MEP pathway to other pathways for supply of glyceraldehyde-3-phosphate and pyruvate, the two starting molecules of MEP pathway. Module 1, that is, glycolysis, is of interest as the overexpressed enzyme glyceraldehyde-3-phosphate dehydrogenase is involved in this pathway. Gene symbols and the enzymes they encode: dxs: DXP synthase; ispC: DXP reductoisomerase; ispD: DXP-ME synthase; ispE: CDP-ME kinase; ispF: MECPP synthase; ispG: HMBPP synthase; ispH: HMBPP reductase; idi: IPP isomerase; ispS: isoprene. Pathway intermediates: G3P: glyceraldehyde-3-phosphate; DXP: 1-deoxy-D-xylulose 5-phosphate; MEP: 2-C-methyl-D-erythritol 4-phosphate; CDP-ME: 4-diphosphocytidyl-2-C-methyl-D-erythritol; CDP-MEP: 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate; MECPP: 2-C-methyl-D-erythritol 2,4-cyclopyrophosphate; HMBPP: 1-hydroxy-2-methyl-2-(E)-butenyl 4-pyrophosphate; IPP: isopentenyl pyrophosphate; DMAPP: dimethylallyl pyrophosphate; DHAP: dihydroxyacetone 3-phosphate.