New Insights into c-Ret Signalling Pathway in the Enteric Nervous System and Its Relationship with ALS
Figure 2
Confocal microscopy. PGP9.5-ir, GFAP-ir, and c-Kit-ir cells in column 1, rows (a) and (b), (c), and (d), respectively; c-Ret-ir in column 2 and merged images in column 3. Example of PGP9.5/c-Ret colocalization; adult enteric neurons labelled with PGP9.5 (a)-1, c-Ret (a)-2, and merged image (a)-3. However, this colocalization does not appear to always occur; PGP9.5-ir neurons (b)-1 does not colocalize with c-Ret neurons (b)-2 within the same ganglia (b)-3. GFAP-ir (c)-1 and c-Ret-ir cells (c)-2 are distinct cells (c)-3. Also, c-Kit-ir cells (d)-1 are c-Ret negatives (d)-2. Overlay image shows no colocalization between the two proteins (d)-3. Nuclear staining (blue). The scale bar is 20 µm in length and applies to all images. 168 228 (300 300 DPI).
