Research Article
Gla-Rich Protein Is a Potential New Vitamin K Target in Cancer: Evidences for a Direct GRP-Mineral Interaction
Table 3
Primers for PCR amplifications.
| | Primer name | Sequence (5′ to 3′) | Purpose/amplicon |
| | dT-adapter | ACGCGTCGACCTCGAGATCGATG(T)13 | RT |
| | A | GTCCCCCAAGTCCCGAGATGAGG | qPCR/GRP-F1 | | B | CCTCCACGAAGTTCTCAAATTCATTCC |
| | C | TCCTGGACGGAGCCCCTA | qPCR/GRP-F5, F6 |
| | D | GCTTCTGCCTGTTTTCCACTTCAC | qPCR/GRP-F5 |
| | E | GCTTCTGCCTGTTTTCCATAGACA | qPCR/GRP-F6 |
| | MGP_F | TGGAGGCTGGCACCTGATTTTG | qPCR/MGP | | MGP_R | AAAAGGGGTGCAGCCAGACAAG |
| | GGCX_F | TTACACAGAGTCGGCGATGGAAGGAT | qPCR/GGCX | | GGCX_R | AGTGTGGTTGTCTAGGCTGCTCTTGAT |
| | VKORC1_F | AGGGCAAGGCTAAGAGGCACTGAG | qPCR/VKORC1 | | VKORC1_R | CTGGGCAATGGAAAGAGCTTTGGAGAC |
| | OPN_F | ACGGACCTGCCAGCAACCGAAGT | qPCR/OPN | | OPN_R | TACTGGATGTCAGGTCTGCGAAA |
| | TNF_F | AGGGCCTGTACCTCATCTACTCCCA | qPCR/TNF | | TNF_R | AGCTGGAAGACCCCTCCCAGATAGA |
| | 18S_F | GGAGTATGGTTGCAAAGCTGA | qPCR/18S | | 18S_R | GGAGTATGGTTGCAAAGCTGA |
| | GAPDH_F | AAGGTGAAGGTCGGAGTCAACGGA | qPCR/GAPDH | | GAPDH_R | TCGCTCCTGGAAGATGGTGATGGG |
| | HsGRPCDS1_Fw | GACGCCTGGTCTGCCTTGTGGG | cDNA cloning |
| | HsGRPCDS3_Rv | CTTGGGAACGAAGCCAGGGGA | cDNA cloning |
| | HsGRPCDS2_Fw | TCCTGGACGGAGCCCCTACCT | cDNA cloning |
| | HsGRPEx5R2 | ACACGGGGATGCCAATGGTGCTAC | cDNA cloning |
| | ReHsGRP_CFw |
*
CACCTCCCCCAAGTCCCGAGATGA | pET151/D-TOPO cloning |
| | ReHsGRP_Rv | TCACGTGTGGTGGCGGTTGTAGA | pET151/D-TOPO cloning |
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Overhang sequence to pET151/D-TOPO for directional cloning.
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