Research Article
A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus
Table 5
Comparison of characteristics of BSH with B. edulis lectin.
| Characteristics | Boletus speciosus hemagglutinin | B. edulis lectin [11] |
| Chromatographic behavior | | | DEAE ion exchanger | Unadsorbed | Unadsorbed | Q ion exchanger | Adsorbed | NA | SP ion exchanger | Adsorbed | NA | CM ion exchanger | Adsorbed | Adsorbed | Molecular mass (kDa) | 31 | 32.6 | Subunit molecular mass (kDa) | 15.5 | 16.3 | N-terminal amino acid sequence | ANVKIVK | TYGIALRV | Thermostability | 60°C | 60°C | pH stability | <12.5 mM NaOH; <6.25 mM HCl | <25 mM NaOH; <25 mM HCl | Sugar specificity | Unknown | Xylose, melibiose | Effect of cations on hemagglutinating activity | Inhibited by Hg2+, Fe2+, Ca2+, and ions; no augmentation observed | inhibited by Mg2+, Mn2+, and Zn2+ ions; augmented by Fe3+ and Al3+ ions | Antifungal activity | Undetectable | Undetectable | Antiproliferative activity | IC50 of 4.7–7.0 M | NA | HIV-1 RT inhibitory activity | IC50 of 7.1 M | IC50 of 14.3 M |
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NA: not available.
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