A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus

Table 5

Comparison of characteristics of BSH with B. edulis lectin.

Characteristics	Boletus speciosus hemagglutinin	B. edulis lectin [11]
Chromatographic behavior
DEAE ion exchanger	Unadsorbed	Unadsorbed
Q ion exchanger	Adsorbed	NA
SP ion exchanger	Adsorbed	NA
CM ion exchanger	Adsorbed	Adsorbed
Molecular mass (kDa)	31	32.6
Subunit molecular mass (kDa)	15.5	16.3
N-terminal amino acid sequence	ANVKIVK	TYGIALRV
Thermostability	60°C	60°C
pH stability	<12.5 mM NaOH; <6.25 mM HCl	<25 mM NaOH; <25 mM HCl
Sugar specificity	Unknown	Xylose, melibiose
Effect of cations on hemagglutinating activity	Inhibited by Hg^2+, Fe^2+, Ca^2+, and Pb^2+𝑖 ions; no augmentation observed	inhibited by Mg^2+, Mn^2+, and Zn^2+ ions; augmented by Fe^3+ and Al^3+ ions
Antifungal activity	Undetectable	Undetectable
Antiproliferative activity	IC50 of 4.7–7.0 𝜇M	NA
HIV-1 RT inhibitory activity	IC50 of 7.1 𝜇M	IC50 of 14.3 𝜇M

NA: not available.