Soybean Seeds: A Practical Host for the Production of Functional Subunit Vaccines
Figure 1
Gene construct design. (a) The pPTN ST108 binary vector used for Agrobacterium-mediated transformation comprising the following regulatory elements: 7S soybean β-conglycinin promoter (P-7S), tobacco etch virus translational enhancer element (TEV), native SEB bacterial signal peptide (SP-N), mutant SEB gene (mSEB), and 35S cauliflower mosaic virus terminator element (T-35s) followed by the selectable marker cassette (nopaline synthase promoter (P-nos), phosphinothricin acetyltransferase gene (bar), and nopaline synthase terminator element (T-nos)). (b) The pPTN 764 binary vector contained soybean 11S glycinin promoter (P-Gly), soybean glycinin signal peptide (SP-Gly), mSEB, and T-35S, followed by the selectable marker cassette. Arrows show orientation of cassettes relative to the right border (RB) and left border (LB) sequences.
