Soybean Seeds: A Practical Host for the Production of Functional Subunit Vaccines
Figure 4
Characterization of soybean-derived mSEB. (a) and (b) Western blot analysis of soy-mSEB, E. coli-derived mSEB, and native SEB under nonreducing and reducing conditions. The ST108 soy-mSEB fragments detected under reducing conditions are labeled I and II, while those derived from 764 soy-mSEB are labeled III and IV. (c) and (d) N-terminal sequencing of soy-mSEB fragments detected under reducing conditions. Amino acids identified from N-terminal protein sequencing are shown in shaded boxes and aligned with the relevant portion of the mSEB protein sequence. The bacterial and soybean signal peptide sequences are underlined with bold typeface. Solid arrows indicate the predicted location for signal peptide cleavage and open arrows indicate observed N-termini. (e) and (f) SignalP 4.1 analysis of the ST108 and 764 soy-mSEB amino acid sequences.
