BioMed Research International

Review Article

Epigenetic Control of Autophagy by MicroRNAs in Ovarian Cancer

Table 1

(a) Autophagy-related genes and their function identified as targets of the microRNA involved in ovarian cancer development and progression (miRanda release, August 2010; TargetScan release 6.2.). (b) Genes coding for autophagy-regulating molecules identified as targets of the microRNA involved in ovarian cancer development and progression (miRanda release, August 2010; TargetScan release 6.2.).

(a)


miRNAs (involved in ovarian cancer progression)	Predicted autophagy Target Genes	Function	Target prediction
miRNAs (involved in ovarian cancer progression)	Predicted autophagy Target Genes	Function	miRanda	TargetScan

hsa-miR-141 hsa-miR 200a	ATG7	A ubiquitin-activating (E1) enzyme homolog that activates both ATG8/LC3 and ATG12	Yes	Yes

hsa-miR-199a -5p	ATG14L	A component of the class III PtdIns 3-kinase complex	No	Yes
	ATG4D	Processing of MAP1-LC3	Yes	Yes
	BECN1	BCL-2 interacting myosin/moesin-like coiled-coil protein 1, part of the class III PtdIns 3-kinase complex (activating macroautophagy)	Yes	Yes

hsa-miR-214	ATG14L	A component of the class III PtdIns 3-kinase complex	No	Yes

hsa-miR-182	ATG7	A ubiquitin-activating (E1) enzyme homolog that activates both ATG8/LC3 and ATG12	Yes	Yes
	ATG16L1	A component of the ATG12-ATG5-ATG16 complex for the formation of autophagosome	Yes	Yes
	MAP1LC3B	Microtubule-associated protein 1 light chain 3, precursor of LC3-II inserted in autophagosomal membranes	Yes	Yes

hsa-miR-140-5p	ATG14L	A component of the class III PtdIns 3-kinase complex	No	Yes

hsa-miR-125b	UVRAG	Interacting with Beclin-1 and Bif-1 (activation and stimulation of macroautophagy)	Yes	Yes

hsa-miR-34a	ATG4B	Processing of MAP1-LC3	Yes	Yes
hsa-miR-34a	ATG9A	A transmembrane protein involved in lipid transport for phagophore expansion	Yes	Yes

hsa-let-7a	ATG4B	Processing of MAP1-LC3	Yes	Yes
	ATG9A	A transmembrane protein involved in lipid transport for phagophore expansion	Yes	Yes
	ATG16L1	A component of the ATG12-ATG5-ATG16 complex for the formation of autophagosome	Yes	Yes

hsa-miR-15a hsa-miR-15b	ATG13	A component of the ULK1 complex needed for ULK1 kinase activity	No	Yes
	ATG9A	A transmembrane protein involved in lipid transport for phagophore expansion	Yes	Yes
	ATG14L	A component of the class III PtdIns 3-kinase complex	No	Yes

hsa-miR-210	ATG7	A ubiquitin-activating (E1) enzyme homolog that activates both ATG8/LC3 and ATG12	Yes	Yes

hsa-miR-449b	ATG4B	Processing of MAP1-LC3	Yes	Yes

(b)


miRNAs (involved in ovarian cancer progression)	Predicted autophagy Target genes	Function	Target prediction
miRNAs (involved in ovarian cancer progression)	Predicted autophagy Target genes	Function	miRanda	TargetScan

hsa-miR-141 hsa-miR 200a	PTEN	Protein/lipid phosphatase that reduces the level of PIP3, thus limiting the activation of AKT	Yes	Yes
hsa-miR-141 hsa-miR 200a	TSC1	Tuberose Sclerosis Complex component that negatively regulates mTOR	Yes	Yes

hsa-miR 200b hsa-miR 200c	PTEN	Protein/lipid phosphatase that reduces the level of PIP3, thus limiting the activation of AKT	Yes	Yes

hsa-miR 21	TSC1	Tuberose Sclerosis Complex component that negatively regulates mTOR	Yes	Yes
hsa-miR 21	BCL2	Interactor of Beclin-1 (represses autophagy) and of BAX (represses apoptosis)	Yes	Yes

hsa-miR-125b	UVRAG	Interacting with Beclin-1 and Bif-1 (activation and stimulation macroautophagy)	Yes	Yes
hsa-miR-125b	BCL2	Interactor of Beclin-1 (represses autophagy) and of BAX (represses apoptosis)	Yes	Yes

hsa-miR-101	MTOR	Mammalian target of rapamycin (kinase) component of MTORC1 (that inhibits autophagy) and of MTORC2 (that phosphorylates Akt)	No	Yes
hsa-miR-101	RAB5A	Endocytic vesicle associated ras-homolog GTPase (involved in autophagosome formation)	Yes	Yes

hsa-miR-31	RAB1B	Endocytic vesicle associated ras-homolog GTPase (involved in autophagosome formation)	Yes	Yes

hsa-miR-34a	BCL2	Interactor of Beclin-1 (represses autophagy) and of BAX (represses apoptosis)	Yes	Yes

has-let-7a	TSC1	Tuberose Sclerosis Complex component that negatively regulates mTOR	Yes	Yes

hsa-miR-15a hsa-miR-15b	BCL2	Interactor of BECLIN 1 (represses autophagy) and of BAX (represses apoptosis)	Yes	Yes
	TSC1	Tuberose Sclerosis Complex component that negatively regulates mTOR	Yes	Yes
	FKBP1A	An immunophilin that forms a complex with rapamycin and inhibits mTOR activity	Yes	Yes

hsa-miR-155	PDK1	Kinase that phosphorylates AKT in Thr308	Yes	Yes
hsa-miR-155	RPTOR	Regulatory associated protein of mTOR (component of MTORC1)	No	Yes

hsa-miR-99a hsa-miR-100	MTOR	Mammalian target of rapamycin (kinase) component of MTORC1 (that inhibits autophagy) and of MTORC2 (that phosphorylates Akt)	No	Yes

hsa-miR-449b	BCL2	Interactor of Beclin-1 (represses autophagy) and of BAX (represses apoptosis)	Yes	Yes