Streptomyces flavogriseus HS1: Isolation and Characterization of Extracellular Proteases and Their Compatibility with Laundry Detergents

Table 3

Effect of various enzyme inhibitors (5 mM) on the activity of extracellular proteases from Streptomyces  flavogriseus HS1.

Inhibitors	Remaining activity (%)
None	100
PMSF	5
EDTA	23

The secreted proteases from Streptomyces  flavogriseus HS1 were preincubated with various enzyme inhibitors for 30 min at room temperature and the remaining activity was determined at pH 7.0 and 50°C. Crude protease activity measured in the absence of any inhibitor was taken as 100%.