Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment

Table 2

List of the conditions used in particle bombardment transformation of sago palm explants. The optimized condition includes the number of bombardments, helium pressure, distance of gene gun to targets, and media used for pretreatment of target cells. Twenty-four transformants were generated from four replicates of three trials. The transformants were selected on Basta and subsequently regenerated new callus.

Transformant number	Bombardment conditions				Type of target
	Number of bombardments	Helium pressure	Distance	Media
1	1x	280 psi	8 cm	Plasmolysed	D3
2
3					D0E
4
5					D0E
6					D0E
7	1x	280 psi	6 cm	Plasmolysed	D0E
8
9					D0E
10					D0E
11
12
13					D0E
14					D2
15					D0E
16					D0E
17					D0E
18	2x	280 psi	6 cm	Plasmolysed	D0E
19					D0E
20					D3
21	2x	280 psi	5 cm	Plasmolysed	D0E
22					D0E
23					D2
24					D2

Transformants that could not produce embryogenic calli and embryoid bodies.