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BioMed Research International
Volume 2014 (2014), Article ID 349074, 10 pages
http://dx.doi.org/10.1155/2014/349074
Research Article

Effect of Alocasia indica Tuber Extract on Reducing Hepatotoxicity and Liver Apoptosis in Alcohol Intoxicated Rats

1CSIR-Indian Institute of Chemical Biology, Drug Development and Biotechnology, 4 Raja S. C. Mullick Road, Kolkata 700032, India
2Serampore College, Department of Physiology, Hooghly, West Bengal 712201, India
3Drug Development Diagnostics and Biotechnology Division, CSIR-Indian Institute of Chemical Biology, Government of India, 4 Raja S. C. Mullick Road, Kolkata, West Bengal 700032, India

Received 28 February 2014; Accepted 17 April 2014; Published 29 May 2014

Academic Editor: Kota V. Ramana

Copyright © 2014 Swagata Pal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The possible protective role of ethanolic extract of A. indica tuber (EEAIT) in hepatotoxicity and apoptosis of liver caused by alcohol in rats was investigated. Treatment of rats with alcohol (3 g ethanol per kg body weight per day for 15 days intraperitoneally) produced marked elevation of liver biomarkers such as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), -glutamyl transpeptidase ( -GT), and total bilirubin levels which were reduced by EEAIT in a dose-dependent manner. Furthermore, EEAIT improved antioxidant status (MDA, NO, and GSH) and preserved hepatic cell architecture. Simultaneous supplementation with EEAIT significantly restored hepatic catalase (CAT) and superoxide dismutase (SOD) activity levels towards normal. The studies with biochemical markers were strongly supported by the histopathological evaluation of the liver tissue. EEAIT also attenuated apoptosis and necrosis features of liver cell found in immunohistochemical evaluation. HPLC analysis of the extract showed the presence of three major peaks of which peak 2 (RT: 33.33 min) contains the highest area (%) and UV spectrum analysis identified it as flavonoids. It is therefore suggested that EEAIT can provide a definite protective effect against chronic hepatic injury caused by alcohol in rats, which may mainly be associated with its antioxidative effect.