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BioMed Research International
Volume 2014, Article ID 349182, 7 pages
Research Article

Hematological and Biochemical Markers of Iron Status in a Male, Young, Physically Active Population

1Faculty of Biomedical Sciences, Metrocamp College-IBMEC Group, 13035-270 Campinas, SP, Brazil
2Department of Clinical Pathology, School of Medical Sciences, State University of Campinas, 13083-881 Campinas, SP, Brazil
3Laboratory of Instrumentation for Biomechanics, Physical Education Institute, State University of Campinas, 13083-851 Campinas, SP, Brazil
4Laboratory of Exercise Biochemistry-LABEX, Biochemistry Department, Biology Institute, State University of Campinas, 13083-970 Campinas, SP, Brazil

Received 21 February 2014; Accepted 5 June 2014; Published 22 June 2014

Academic Editor: Patrizia Cardelli

Copyright © 2014 Lázaro Alessandro Soares Nunes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to establish reference intervals (RIs) for the hemogram and iron status biomarkers in a physically active population. The study population included male volunteers ( ) with an average age of 19 ± 1 years who had participated in a regular and controlled exercise program for four months. Blood samples were collected to determine hematological parameters using a Sysmex XE-5000 analyzer (Sysmex, Kobe, Japan). Iron, total iron-binding capacity (TIBC), transferrin saturation and ferritin, and high-sensitivity C-reactive protein (CRP) concentrations in serum samples were measured using commercial kits (Roche Diagnostics, GmbH, Mannheim, Germany) and a Roche/Hitachi 902 analyzer. The RIs were established using the RefVal program 4.1b. The leucocyte count, TIBC, and CRP and ferritin concentrations exhibited higher RIs compared with those in a nonphysically active population. Thirty volunteers (outliers) were removed from the reference population due to blood abnormalities. Among the outliers, 46% exhibited higher CRP concentrations and lower concentrations of iron and reticulocyte hemoglobin compared with the nonphysically active population ( ). Our results showed that it is important to establish RIs for certain laboratory parameters in a physically active population, especially for tests related to the inflammatory response and iron metabolism.