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BioMed Research International
Volume 2014 (2014), Article ID 351204, 8 pages
Research Article

Association of Levels of Antibodies from Patients with Inflammatory Bowel Disease with Extracellular Proteins of Food and Probiotic Bacteria

1Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, 33300 Asturias, Spain
2Department of Functional Biology, Immunology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain
3UMR 5248 CBMN CNRS-Université Bordeaux 1-ENITAB, Laboratoire de Microbiologie et Biochimie Appliquée, 1 Cours du Général de Gaulle, 33175 Gradignan Cedex, France
4Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo, Ourense Campus, 32004 Ourense, Spain

Received 27 February 2014; Revised 18 May 2014; Accepted 19 May 2014; Published 4 June 2014

Academic Editor: Julio Villena

Copyright © 2014 Arancha Hevia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Inflammatory bowel disease (IBD) is an autoimmune disease characterized by a chronic inflammation of the gastrointestinal tract mucosa and is related to an abnormal immune response to commensal bacteria. Our aim of the present work has been to explore the levels of antibodies (IgG and IgA) raised against extracellular proteins produced by LAB and its association with IBD. We analyzed, by Western-blot and ELISA, the presence of serum antibodies (IgA and IgG) developed against extracellular protein fractions produced by different food bacteria from the genera Bifidobacterium and Lactobacillus. We used a sera collection consisting of healthy individuals (HC, ), Crohn's disease patients (CD, ), and ulcerative colitis patients (UC, ). Levels of IgA antibodies developed against a cell-wall hydrolase from Lactobacillus casei subsp. rhamnosus GG (CWH) were significantly higher in the IBD group (; ). The specificity of our measurements was confirmed by measuring IgA antibodies developed against the CWH peptide 365-VNTSNQTAAVSAS-377. IBD patients appeared to have different immune response to food bacteria. This paper sets the basis for developing systems for early detection of IBD, based on the association of high levels of antibodies developed against extracellular proteins from food and probiotic bacteria.