Rapid Purification of a New P-I Class Metalloproteinase from Bothrops moojeni Venom with Antiplatelet Activity
Figure 8
Effect of BmooMPα-II (20, 40, and 80 μg) from B. moojeni venom on (a) ADP, (b) collagen, and (c) ristocetin-induced platelet aggregation. Aggregation was triggered with agonists immediately after adding the indicated doses of BmooMPα-II to human PRP at 37°C. Platelet aggregation was recorded for 10 min in an AggRAM platelet aggregation system with four-channel laser optics (Helena Laboratories, EUA). Results were expressed as an increase in light transmission, where PPP represents the maximum response (100%). Control experiments were performed in the absence of BmooMPα-II.
