Biocatalytic Synthesis of Flavor Ester “Pentyl Valerate” Using Candida rugosa Lipase Immobilized in Microemulsion Based Organogels: Effect of Parameters and Reusability

Table 3

Estimated regression coefficients for esterification (%) for enzyme concentration (𝑋₁), incubation time (𝑋₂), substrate ratio (𝑋₃), Wo (𝑋₄), and solvent for MBG preparation (𝑋₅).

Term	Coefficient	Standard error coefficient (SE)	𝑇	𝑃
X 1	16.7997	1.338	12.557	0.000
X 2	15.1419	1.338	11.318	0.000
X 3	10.4122	1.409	7.390	0.000
X 4	9.9234	1.338	7.418	0.000
X 5	−3.0408	1.390	−2.187	0.033
𝑋^21	−3.7261	4.338	−0.859	0.394
𝑋^22	−7.8221	2.906	−2.692	0.009
𝑋^23	−13.5324	2.257	−5.996	0.000
𝑋^24	−1.1522	2.209	−0.521	0.604
𝑋^25	−3.8029	2.898	−1.312	0.194
X 1X 2	0.1887	2.676	0.071	0.944
X 1X 3	11.5275	2.676	4.308	0.000
X 1X 4	−5.6825	2.676	−2.124	0.038
X 1X 5	−0.7100	2.676	−0.265	0.792
X 2X 3	8.2425	2.676	3.081	0.003
X 2X 4	−2.3162	2.676	−0.866	0.390
X 2X 5	1.8575	2.676	0.694	0.490
X 3X 4	5.8313	2.676	2.179	0.033
X 3X 5	−3.7794	3.072	−1.230	0.223
X 4X 5	2.2137	2.676	0.827	0.411