Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells
Figure 3
MC3T3-E1 proliferation was measured 24 and 72 hours following a single dose of thermal stress (44°C, 4 or 8 minutes, postheating incubation = 4 hours) and cyclic tension (equibiaxial 3%, 0.2 Hz, 10-second tension on/10-second rest, 24 and 72 hours) individually or in combination. Cell proliferation was measured using MTS and PicoGreen DNA assay with varying heating durations of 4 minutes ((a) and (b)) and 8 minutes ((c) and (d)) ().
