Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells
Figure 5
Gene expression and protein secretion of OPN and OPG by MC3T3-E1 cells following a single dose of thermal stress (44°C, 4 or 8 minutes) and cyclic tension (equibiaxial 3%, 0.2 Hz, 10-second tension on/10-second rest, 24 hours or 72 hours) individually or in combination. OPN and OPG gene expression is shown in response to varying heating durations of 4 minutes ((a) and (b)) and 8 minutes ((c) and (d)) alone or in combination with cyclic tension for 24 hours. OPN (e) and OPG (f) concentration secreted in the culture supernatant (measured by ELISA) is shown following heating for 4 minutes and cyclic tension for 24 or 72 hours. denotes statistical significance between stress-treated and sham-treated groups () ().
