Figure 2: Effect of challenge with vasoconstrictor on the mean blood pressure and the expression of musclin protein. Wistar rats were injected intravenously (IV) with a vasoconstrictor named phenylephrine (PE; 10 μg/kg) dissolved in 9% saline, the used vehicle, 4 times daily for one week. The mean blood pressure (MBP) was recorded using a noninvasive tail-cuff monitor (a), while the expression of musclin protein (11 kDa) was determined using Western blotting analysis (b). The quantification of the results is indicated as the means with the SE ( per group) in each column shown in the lower panel. * compared to vehicle-treated group.